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t75 culture flasks  (Greiner Bio)


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    Greiner Bio t75 culture flasks
    T75 Culture Flasks, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 338 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t75 culture flasks/product/Greiner Bio
    Average 94 stars, based on 338 article reviews
    t75 culture flasks - by Bioz Stars, 2026-04
    94/100 stars

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    Schematic representation of the experimental design to investigate the impact of acid stress on oral epithelial cells (OECs) and Toll-like receptor (TLR) agonist stimulation. pH Conditioning : OECs (80% confluence) were cultured in acidified (pH:=:3.0) or complete growth media (pH:=:8.0) for 24h. Morphometric Analysis ( a ): Brightfield micrographs and a machine-learning based image analysis pipeline were used to assess changes in cellular morphology. TLR Agonist Challenge : Cell cultures were subjected to either 100 ng/mL flagellin (TLR5 agonist) or 1 mg/mL Pam3CSK4 (TLR2/1 agonist) for 2-, 6-, or 24h. Molecular profiling ( b ): was conducted on OEC RNA collected after 6h TLR agonist challenge using NanoString® nCounter® technology followed by pathway analysis using the Gene Ontology knowledgebase. A TGF- β ELISA ( c ) was performed on OEC supernatants collected 2-, 6-, and 24h post TLR agonist stimulation. Figure made with Biorender.com

    Journal: bioRxiv

    Article Title: Acid stress modulates metabolo-inflammatory pathways in oral epithelial cells

    doi: 10.64898/2026.03.16.711383

    Figure Lengend Snippet: Schematic representation of the experimental design to investigate the impact of acid stress on oral epithelial cells (OECs) and Toll-like receptor (TLR) agonist stimulation. pH Conditioning : OECs (80% confluence) were cultured in acidified (pH:=:3.0) or complete growth media (pH:=:8.0) for 24h. Morphometric Analysis ( a ): Brightfield micrographs and a machine-learning based image analysis pipeline were used to assess changes in cellular morphology. TLR Agonist Challenge : Cell cultures were subjected to either 100 ng/mL flagellin (TLR5 agonist) or 1 mg/mL Pam3CSK4 (TLR2/1 agonist) for 2-, 6-, or 24h. Molecular profiling ( b ): was conducted on OEC RNA collected after 6h TLR agonist challenge using NanoString® nCounter® technology followed by pathway analysis using the Gene Ontology knowledgebase. A TGF- β ELISA ( c ) was performed on OEC supernatants collected 2-, 6-, and 24h post TLR agonist stimulation. Figure made with Biorender.com

    Article Snippet: Low-passage mixed-donor, Primary Human Oral Epithelial Cells (Cat# 36063-01, Celprogen, Inc., Torrance, USA), were thawed from liquid nitrogen, plated on poly-L-lysine-coated T-75 cell culture flasks (Sigma-Aldrich Co., St. Louis, USA; Corning Inc., Durham, USA) and maintained in Human OEC Culture Complete Growth Media (Celprogen, Inc.) containing serum and antibiotics at 37°C and 5% CO 2 for two passages prior to experimentation according to manufacturer recommendations.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay